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KMID : 0364820150510030288
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Korean Journal of Microbiology
2015 Volume.51 No. 3 p.288 ~ p.299
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Optimal conditions and effects of prebiotics for growth and antimicrobial substances production of Lactobacillus brevis BK11
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Lim Eun-Seo
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Abstract
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Lactobacillus brevis BK11 obtained from Baikkimchi was selected to study the effects of culture medium, initial pH,
atmosphere composition, incubation temperature and time, and prebiotics on growth and production of antimicrobial substances.Growth and antimicrobial substances production of L. brevis BK11 were significantly higher in MRS broth than in BHI or M17 broth. Theproduction of cell mass, lactic acid, and bacteriocin by BK11 strain was at maximum in MRS broth adjusted to pH 6.0. Aerobic andmicroaerobic conditions were favored cell growth and antimicrobial substances production than anaerobic condition. Biomass and lacticacid production and antimicrobial substances activity of BK 11 were significantly better at 30 and 37°C than at 25°C. Growth of the strainBK11 entered the stationary growth stage at 24 h after inoculation, and decreased after 36 h. Antimicrobial activities of cell-free culturesupernatant and bacteriocin solution were highest when cultured in MRS broth with an initial pH 6.0 for 24?30 h at 37°C. In addition, the highest cell number and lactic acid and bacteriocin production were recorded in the presence of 1 and 2% (w/v) fructooligosaccharide (FOS), however, inulin and raffinose did not affect biological and physicochemical characteristics and antimicrobial activities of L. brevis BK11 cultures. According to these results, production of antimicrobial substances by L. brevis KB11 was closely associated with cell density. Under optimal conditions for antimicrobial substances production, L. brevis BK11 effectively inhibited the growth of Helicobacter pylori ATCC 43504.
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KEYWORD
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Helicobacter pylori, antimicrobial activity, bacteriocin, prebiotic, probiotic
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